Hydrocortisone

Cell Culture Grade

CAS Number                        50-23-7

Formula                                 C₂1H₁0O₅

Molecular Weight                362.46

Assay                                      ≥ 98%

Form                                       Powder

Melting Point                         211 – 214^oC

Reconstitution:

To prepare 50 μg/mL stock solutions; add 1.0 mL absolute ethanol to 1.0 mg of product, gently swirl to dissolve, add 19 mL sterile medium.

Physical form:

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw.

Application:

Hydrocortisone has been used as a supplement in culture media for oligodendrocytes, colon cells and epithelial cells.

Biochem/physiol Actions:

Hydrocortisone (also referred to as cortisol) is an endogenous glucocorticoid which is secreted by the adrenal gland. It is produced from the hypothalamus–pituitary–adrenal (HPA) axis. Hydrocortisone interacts with glucocorticoid receptors and regulates various signaling pathways. It is associated with the induction of energy mobilization, increase in cerebral perfusion rates and local glucose consumption. It is also linked with increase in cardiovascular output and respiration, redistribution of blood, delivery of molecules and energy to the brain and muscles and modulation of immune responses.

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na²⁺ retention potency.